RIKEN Seminar
The 3rd Annal Meeting of Structual-Biological Whole Cell Project of Thermus thermophilus HB8
July 30 (Fri) to August 1 (Sun), 2004
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Yuki Maruyama, Hidetoshi Yamashita, Toru Suzuki, Ryuta Komatsu, Masanari Kitagawa
(DragonGenomicsCenter, TakaraBio Inc.)
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Ryoji MASUI1,2, Ken KUROKAWA3, Noriko NAKAGAWA1,2, Takaho TERADA4, Mikako SHIROUZU4, Yoshinori KOYAMA5, Fumio TOKUNAGA1, Tairo OSHIMA6, Teruo YASUNAGA3, Kunio MIKI2,7, Shigeyuki YOKOYAMA2,4, Seiki KURAMITSU1,2,4
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst., 3Genomic Information Res. Cent., Osaka Univ., 4RIKEN GSC, 5Nat. Inst. Biosci. Human Tech., MITI, 6Grad. Sch. Life Sci., Tokyo Univ. Pharm., 7Grad. Sch. Sci., Kyoto Univ.)
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3. Protein Expression
Nobuko MAOKA, Yukihide KOUSUMI, Chizu KUROISHI et al.
(RIKEN Harima Inst.)
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4. Protein Purification
Nobuko MAOKA, Chizu KUROISHI et al.
(RIKEN Harima Inst.)
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5. Purification Report 1
Fujie SHINKAI, Nobuko MAOKA et al.
(RIKEN Harima Inst.)
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6. Purification Report 2
Miwa OHMORI, Masami NISHIDA et al.
(RIKEN Harima Inst.)
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7. Purification Report 3
Yumiko INOUE, Miho MANZOKU et al.
(RIKEN Harima Inst.)
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8. Purification Report 4
Emiko MATSUNAGA, Yayoi FUJIMOTO et al.
(RIKEN Harima Inst.)
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9. Purification Report 5
Noriko ITO, Yoko UKITA et al.
(RIKEN Harima Inst.)
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10. Protein Crystallization
Hitoshi IINO et al.
(RIKEN Harima Inst.)
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11. List of Structural and Functional Analyses
Mayumi KANAGAWA et al.
(RIKEN Harima Inst.)
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12. DNA Microarray Analysis
Aiko KASHIHARA et al.
(RIKEN Harima Inst.)
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13. Database System for Managing and Sharing Experiment Information of Thermus thermophilus HB8
Yoshihiro AGARI, Satoshi KIRA et al.
(RIKEN Harima Inst.)
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Akinobu FUKUZAKI1,2, Fumikazu KONISHI1,2, Takeshi NAGASHIMA1, Kaori IDE1,2, Mariko HATAKEYAMA1,2, Seiki KURAMITSU2,3, Akihiko KONAGAYA1,2
(1RIKEN GSC, 2RIKEN Harima Inst, 3Grad. Sch. Sci., Osaka Univ.)
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Shuhei Kimura1,2, Mariko Hatakeyama1,2, Akihiko Konagaya1,2
(1RIKEN GSC, 2RIKEN Harima Inst.)
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17. N-Terminal Protein Sequencing Procedure Using Bis(terpyridine)ruthenium(II)
derivative
Ken Masui1, Taka-aki Okamura1, Hitoshi Yamamoto1,2, Norikazu Ueyama1
(1Grad. Sch. Sci., Osaka Univ., 2Riken Harima Inst.)
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Kim S.-H.1, Miyatake H.1, Ueno T.2, Nagao T.2, and Miki K.1,3
(1RIKEN Harima Inst., 2Tecan Japan, 3Grad. Sch. Sci.,Kyoto Univ.)
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Katsuhide Yutani1, Masahide Sawano1, Michiyo Takehira1, Junji Nakamura2, Tomoyuki Tanaka1, Yoko Ukita1, and Shigeyuki Yokoyama1,3,4
(1RIKEN Harima Inst., 2Nihon SiberHegner KK, 3RIKEN GSC, 4Grad. Sch. Sci., Univ. Tokyo)
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Hideyuki Miyatake1, Seong-Hoon Kim1, Itsuro Motegi2, Hirofumi Matsuzaki2, Hideyoshi Kitahara2, Akira Higuchi2, Kunio Miki1,3
(1RIKEN Harima Inst., 2Panasonic Factory Solutions Co., 3Grad. Sch. Sci., Kyoto Univ.)
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Go Ueno1,2, Raita Hirose3, Koh Ida1, Takashi Kumasaka1,4, Masaki Yamamoto1
(1RIKEN Harima Inst., 2Rigaku Co., 3PharmAxess Inc., 4Tokyo Institute of Technology)
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Min Yao, Yong Zhou, Isao Tanaka
(Grad. Sch. Sci., Hokkaido Univ.)
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Yukuhiko Asada1, Hideyuki Taka1,2, Michihiro Sugahara1, Haruhiko Ayama1, Hisashi Ukawa1,2, Naoki kunishima1
(1RIKEN Harima Inst., 2Hitachi Software Engineering Co. Ltd.)
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Michihiro Sugahara, Yuichi Nodake, Mitsuaki Sugahara, Naoki Kunishima
(RIKEN Harima Inst.)
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Bagautdin Bagautdinov, Yayoi Fujimoto, Tahir H Tahirov
(RIKEN Harima Inst.)
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Ikuko Miyahara1,2, Mitsuyoshi Matsumura1,2, Masaru Goto1, Rie Omi1, Ken Hirotsu1,2
(1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)
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28. X-ray Structures of Threonine dehydrogenase Complexes
Tadayuki Yao1,2, Rie Omi1, Masaru Goto1, Ikuko Miyahara1,2, Ken Hirotsu1,2
(1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)
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29. Crystal Structure of Histidinol Phosphate Phosphatase from Thermus thermophilus HB8
Rie Omi1, Masaru Goto1, Ikuko Miyahara1,2 and Ken Hirotsu1,2
(1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)
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30. Substrate-recognition mechanism of homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus, as revealed by crystallographic and mutational analysis
Junichi Miyazaki1, Shinya Fushinobu2, Makoto Nishiyama1
(1Biotechnol. Res. Center., Tokyo Univ., 2Grad. Sch. Biotech., Tokyo Univ.)
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31. Structural and Functional Analysis of NDP Kinase from Thermus thermophilus HB8
Shunsaku TAKEISHI1, Noriko NAKAGAWA1,2, Ryoji MASUI1,2, and Seiki KURAMITSU1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Gen-ichi Sampei1,2, Seiki Baba3, Takeshi Ishii1, Hiroya Kawai3, Hisaaki Yanai1,2, Mayumi Kanagawa2,3, and Gota Kawai2,3
(1Dept. Applied Physics & Chemistry, Univ. Electro-Communications, 2RIKEN Harima Inst., 3Fac. Eng., Chiba Inst. Tech.)
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Takeshi Ishii1, Seiki Baba2, Mayumi Kanagawa2,3, Hisaaki Yanai1,3, Hiroya Kawai2, Akio Ebihara3, Noriko Nakagawa3, Gota Kawai2,3 and Gen-ichi Sampei1,3
(1Dept. Applied Physics & Chemistry, Univ. Electro-Communications, 2Fac. Eng., Chiba Inst. Tech., 3RIKEN Harima Inst.)
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Shinji SUEDA1, Md. Nurul Islam1, Jin Yong-Biao1, Hiroki Kondo1,2
(1Kyushu Inst. Tech., 2RIKEN Harima Inst.)
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35. Reaction mechanism of GTP cyclohydrolase I
Yoko TANAKA1,2, Noriko NAKAGAWA1,2, Ryoji MASUI1,2 and Seiki KURAMITSU1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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36. Crystal Structure of Dihydroneopterin Aldolase from Thermus thermophilus HB8
Tamao Hisano1, Shinya Satoh1, Kunio Miki1,2
(1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)
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37. X-ray Structure of Thermus thermophilus HB8 L-Aspartate-a-Decarboxylase at Atomic Resolution
Osamu Nakajima1,2, Masaru Goto1, Rie Omi1, Ikuko Miyahara1,2, Ken Hirotu1,2
(1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)
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38. Crystallization and Preliminary Crystallographic Analysis of Short-Chain Dehydrogenase/Reductase (SDR) from Thermus thermophilus HB8
Tomoyasu Kichise1, Kazuki Takeda1, Tamao Hisano1, Kunio Miki1,2
(1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)
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Nobutaka Numoto1, Akiko Kita1, Kunio Miki1,2
(1Grad. Sch. Sci., Kyoto Univ., 2RIKEN Harima Inst.)
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40. Inhibition of Thermus thermophilus HB8 Thioredoxin Activity by Platinum(II) Complex
Masahiro Kato1, Hitoshi Yamamoto1,2, Taka-aki Okamura1, Ryoji Masui1,2, Seiki Kuramitsu1,2, Norikazu Ueyama1
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Hiroshi Hashimoto, Naoki Shichijo, Toshiyuki Shimizu, Mamoru Sato
(Grad. Sch. Integrated Sci., Yokohama City Univ.)
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43. Crystal structure of P-protein of the glycine cleavage system from Thermus thermophilus HB8
Tadashi Nakai1,2, Noriko Nakagawa1,2, Nobuko Maoka1, Ryoji Masui1,2, Seiki Kuramitsu1,2, Nobuo Kamiya1
(1RIKEN Harima Inst., 2Grad. Sch. Sci., Osaka Univ.)
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Shintaro Misaki1, Kenji Suzuki1, Naoki Kunishima2, Mitsuaki Sugawara2, Chizu Kuroishi2, Masanori Kobayashi1, Shoko Fujimoto1, Masahiro Sakurai1, Kazumi Nishijima1
(1Joint Research Group, Pharmaceutical Consortium for Protein Structure Analysis (PcProt), 2RIKEN Harima Inst.)
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45. Crystallographic analysis of malate dehydrogenase from thermophilic bacterium Thermus flavus and the mutant EX7 to elucidate the molecular mechanisms of their cofactor specificities and catalytic properties
Takeo Tomita1, Shinya Fushinobu2, Makoto Nishiyama1
(1Biothch. Res. Center., Tokyo Univ., 2Grad. Sch. Biotech., Tokyo Univ.)
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Shiho TSUTSUI1, Woo Cheol LEE1, Kosuke ITO1, Masayuki KAMO1, Yumiko INOUE2, Koji NAGATA1, Masaru TANOKURA1,2
(1Grad. Sch. Agr. and Life Sci., Tokyo Univ., 2RIKEN Harima Inst.)
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Masayuki Kamo1, Norio Kudo1, WooCheol Lee1, Hiroyuki Motoshima2, Masaru Tanokura1,2
(1Grad. Sch. Agr. and Life Sci. Tokyo Univ., 2RIKEN Harima Inst.)
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48. X-Ray Analysis of Peptideglycan Synthetase family from Thermus thermophilus HB8
Shohei Kobayashi1, Fujio Matsuzaki1, Ryoko Hirooka1, Makoto Kimura1,2, Yoshimitsu Kakuta1,2
(1Grad. Sch. Agr., Kyushu Univ., 2RIKEN Harima Inst.)
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49. Crystallographic studies of an ABC-ATPase (TT2939) from T. thermophilus HB8
Kazuki Takeda1, Kunio Miki1,2
(1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)
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50. Crystal structure of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8
Wakana Iwasaki1, Hideyuki Miyatake1 and Kunio Miki1,2
(1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)
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Kenji FUKUI1, Ryoji MASUI1,2, and Seiki KURAMITSU1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Ikumi SAITO1, Noriko NAKAGAWA2, Ryoji MASUI1,2, Seiki KURAMITSU1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Hitomi Yamaguchi, Kei Wada, Atsushi Yamagata, Yasuhiro Takahashi and Keiichi Fukuyama
(Grad. Sch. Sci., Osaka Univ.)
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76. The Crystal Structure of Thermus thermophilus HB8 hypothetical protein TT1253
Chizuko Sasaki1, Ikuko Sugiura1, Shigetoshi Sugio1, Takashi Tamura2,3, Kenji Inagaki2,3
(1Mitsubishi Chem. Co., 2RIKEN Harima Inst., 3Grad. Sch.Natural Sci. and Tech., Okayama Univ.)
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Naoyuki Kondo1, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Takushi Ooga1, Sachiko Yoshiba1, Noriko Nakagawa1, Ryoji Masui1,2 and Seiki Kuramitsu1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Takashi Nishikubo1, Ryoji Masui1,2, Seiki Kuramitsu1,2
(1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)
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Taisuke Wakamatsu1, Takushi Ooga2, Noriko Nakagawa2, Ryoji Masui2,3 and Seiki Kuramitsu1,2,3
(1Grad. Sch. Frontier Biosci., Osaka Univ ., 2Grad. Sch. Sci., Osaka Univ ., 3RIKEN Harima Inst.)
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Daisuke Yamagishi1, Ryoji Masui1,2 and Seiki Kuramitsu1,2
(1Grad. Sch. Sci., Osaka Univ. 2RIKEN Harima Inst.)
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Mio Ohnuma1, Yusuke Terui1, Mizue Nagai1, Hiromi Ito1, Aya Chitose1, Masatada Tamakoshi1, Shin-ichi Yokobori1, Masaru Niitsu2, Keijiro Samejima2, Tairo Oshima1
(1Sch. Life Sci., Tokyo Univ Pharm. Life Sci., 2Sci. Facl. Pharm., Josai Univ.)
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83. Crystal structures of the signal transducing protein GlnK from Thermus thermophilus HB8
Hiroaki Sakai1, Hongfei Wang1, Chie Takemoto-Hori1, Tatsuya Kaminishi1,2, Hiroto Yamaguchi1, Yuki Kamewari1, Takaho Terada1,3, Seiki Kuramitsu1,3,4, Mikako Shirouzu1,4 and Shigeyuki Yokoyama1,2,4
(1RIKEN GSC, 2Grad. Sch. Sci., Tokyo Univ., 3Grad. Sch. Sci., Osaka Univ., 4RIKEN Harima Inst.)
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