RIKEN Seminar

The 3rd Annal Meeting of Structual-Biological Whole Cell Project of Thermus thermophilus HB8

July 30 (Fri) to August 1 (Sun), 2004



      

1. The Software for Viewing the Genome Sequence and Annotations of Thermus thermophilus HB8 

Yuki Maruyama, Hidetoshi Yamashita, Toru Suzuki, Ryuta Komatsu, Masanari Kitagawa
(DragonGenomicsCenter, TakaraBio Inc.)


2-13. Progress Reports on the Whole Cell Project of Thermus thermophilus HB8 in RIKEN Harima Institute

2. Comparative Analaysis of Thermus thermophilus HB8 Proteins

Ryoji MASUI1,2, Ken KUROKAWA3, Noriko NAKAGAWA1,2, Takaho TERADA4, Mikako SHIROUZU4, Yoshinori KOYAMA5, Fumio TOKUNAGA1, Tairo OSHIMA6, Teruo YASUNAGA3, Kunio MIKI2,7, Shigeyuki YOKOYAMA2,4, Seiki KURAMITSU1,2,4
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst., 3Genomic Information Res. Cent., Osaka Univ., 4RIKEN GSC, 5Nat. Inst. Biosci. Human Tech., MITI, 6Grad. Sch. Life Sci., Tokyo Univ. Pharm., 7Grad. Sch. Sci., Kyoto Univ.)


3. Protein Expression 

Nobuko MAOKA, Yukihide KOUSUMI, Chizu KUROISHI et al.
(RIKEN Harima Inst.)


4. Protein Purification 

Nobuko MAOKA, Chizu KUROISHI et al.
(RIKEN Harima Inst.)


5. Purification Report 1 

Fujie SHINKAI, Nobuko MAOKA et al.
(RIKEN Harima Inst.)


6. Purification Report 2 

Miwa OHMORI, Masami NISHIDA et al.
(RIKEN Harima Inst.)


7. Purification Report 3 

Yumiko INOUE, Miho MANZOKU et al.
(RIKEN Harima Inst.)


8. Purification Report 4 

Emiko MATSUNAGA, Yayoi FUJIMOTO et al.
(RIKEN Harima Inst.)


9. Purification Report 5 

Noriko ITO, Yoko UKITA et al.
(RIKEN Harima Inst.)


10. Protein Crystallization 

Hitoshi IINO et al.
(RIKEN Harima Inst.)


11. List of Structural and Functional Analyses 

Mayumi KANAGAWA et al.
(RIKEN Harima Inst.)


12. DNA Microarray Analysis 

Aiko KASHIHARA et al.
(RIKEN Harima Inst.)


13. Database System for Managing and Sharing Experiment Information of Thermus thermophilus HB8 

Yoshihiro AGARI, Satoshi KIRA et al.
(RIKEN Harima Inst.)


14. Introduction to web based annotation system for Structural-Biological Whole Cell Project Thermus thermophilus HB8

Akinobu FUKUZAKI1,2, Fumikazu KONISHI1,2, Takeshi NAGASHIMA1, Kaori IDE1,2, Mariko HATAKEYAMA1,2, Seiki KURAMITSU2,3, Akihiko KONAGAYA1,2
1RIKEN GSC, 2RIKEN Harima Inst, 3Grad. Sch. Sci., Osaka Univ.)


15. Inference of Genetic Networks using a Cooperative Coevolutionary Algorithm

Shuhei Kimura1,2, Mariko Hatakeyama1,2, Akihiko Konagaya1,2
1RIKEN GSC, 2RIKEN Harima Inst.)


17. N-Terminal Protein Sequencing Procedure Using Bis(terpyridine)ruthenium(II) derivative

Ken Masui1, Taka-aki Okamura1, Hitoshi Yamamoto1,2, Norikazu Ueyama1
1Grad. Sch. Sci., Osaka Univ., 2Riken Harima Inst.)


18. Development of novel ampholyte for three-dimensional structural analysis in the free-flow isoelectric focusing

Kim S.-H.1, Miyatake H.1, Ueno T.2, Nagao T.2, and Miki K.1,3
1RIKEN Harima Inst., 2Tecan Japan, 3Grad. Sch. Sci.,Kyoto Univ.)


19. Checking up on intact protein samples using DSC

Katsuhide Yutani1, Masahide Sawano1, Michiyo Takehira1, Junji Nakamura2, Tomoyuki Tanaka1, Yoko Ukita1, and Shigeyuki Yokoyama1,3,4
1RIKEN Harima Inst., 2Nihon SiberHegner KK, 3RIKEN GSC, 4Grad. Sch. Sci., Univ. Tokyo)


20. Development of full-automated macromolecular crystallization/observation robot system HTS-80

Hideyuki Miyatake1, Seong-Hoon Kim1, Itsuro Motegi2, Hirofumi Matsuzaki2, Hideyoshi Kitahara2, Akira Higuchi2, Kunio Miki1,3
1RIKEN Harima Inst., 2Panasonic Factory Solutions Co., 3Grad. Sch. Sci., Kyoto Univ.)


21. Sample management system of frozen crystals at the SPring-8 RIKEN Structural Genomics Beamlines

Go Ueno1,2, Raita Hirose3, Koh Ida1, Takashi Kumasaka1,4, Masaki Yamamoto1
1RIKEN Harima Inst., 2Rigaku Co., 3PharmAxess Inc., 4Tokyo Institute of Technology)


23. Lafire: an automatic refinement program for protein crystallography

Min Yao, Yong Zhou, Isao Tanaka
(Grad. Sch. Sci., Hokkaido Univ.)


24. Automated crystallographic supporting system for high-throughput protein structure determination

Yukuhiko Asada1, Hideyuki Taka1,2, Michihiro Sugahara1, Haruhiko Ayama1, Hisashi Ukawa1,2, Naoki kunishima1
1RIKEN Harima Inst., 2Hitachi Software Engineering Co. Ltd.)


25. Crystal structure of dehydroquinate synthase from Thermus thermophilus HB8

Michihiro Sugahara, Yuichi Nodake, Mitsuaki Sugahara, Naoki Kunishima
(RIKEN Harima Inst.)


26. Crystal Structure Of Indole-3-Glycerol Phosphate Synthase From Thermus thermophilus HB8

Bagautdin Bagautdinov, Yayoi Fujimoto, Tahir H Tahirov
(RIKEN Harima Inst.)


27. Double Substrate Recognition of Acetylornithine Aminotransferase

Ikuko Miyahara1,2, Mitsuyoshi Matsumura1,2, Masaru Goto1, Rie Omi1, Ken Hirotsu1,2
1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)


28. X-ray Structures of Threonine dehydrogenase Complexes

Tadayuki Yao1,2, Rie Omi1, Masaru Goto1, Ikuko Miyahara1,2, Ken Hirotsu1,2
1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)


29. Crystal Structure of Histidinol Phosphate Phosphatase from Thermus thermophilus HB8

Rie Omi1, Masaru Goto1, Ikuko Miyahara1,2 and Ken Hirotsu1,2
1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)


30. Substrate-recognition mechanism of homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus, as revealed by crystallographic and mutational analysis

Junichi Miyazaki1, Shinya Fushinobu2, Makoto Nishiyama1
1Biotechnol. Res. Center., Tokyo Univ., 2Grad. Sch. Biotech., Tokyo Univ.)


31. Structural and Functional Analysis of NDP Kinase from Thermus thermophilus HB8

Shunsaku TAKEISHI1, Noriko NAKAGAWA1,2, Ryoji MASUI1,2, and Seiki KURAMITSU1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


32. Crystallographic analysis of proteins in the purine nucleotide biosynthetic pathway

Gen-ichi Sampei1,2, Seiki Baba3, Takeshi Ishii1, Hiroya Kawai3, Hisaaki Yanai1,2, Mayumi Kanagawa2,3, and Gota Kawai2,3
1Dept. Applied Physics & Chemistry, Univ. Electro-Communications, 2RIKEN Harima Inst., 3Fac. Eng., Chiba Inst. Tech.)


33. Crystal structure of GMP synthetase from Thermus thermophilus HB8

Takeshi Ishii1, Seiki Baba2, Mayumi Kanagawa2,3, Hisaaki Yanai1,3, Hiroya Kawai2, Akio Ebihara3, Noriko Nakagawa3, Gota Kawai2,3 and Gen-ichi Sampei1,3
1Dept. Applied Physics & Chemistry, Univ. Electro-Communications, 2Fac. Eng., Chiba Inst. Tech., 3RIKEN Harima Inst.)


34. Three-Dimensional Sturcture and Reaction Mechanism of Biotin-Dependent Enzymes

Shinji SUEDA1, Md. Nurul Islam1, Jin Yong-Biao1, Hiroki Kondo1,2
1Kyushu Inst. Tech., 2RIKEN Harima Inst.)


35. Reaction mechanism of GTP cyclohydrolase I

Yoko TANAKA1,2, Noriko NAKAGAWA1,2, Ryoji MASUI1,2 and Seiki KURAMITSU1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


36. Crystal Structure of Dihydroneopterin Aldolase from Thermus thermophilus HB8

Tamao Hisano1, Shinya Satoh1, Kunio Miki1,2
1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)


37. X-ray Structure of Thermus thermophilus HB8 L-Aspartate-a-Decarboxylase at Atomic Resolution

Osamu Nakajima1,2, Masaru Goto1, Rie Omi1, Ikuko Miyahara1,2, Ken Hirotu1,2
1Grad. Sch. Sci., Osaka City Univ., 2RIKEN Harima Inst.)


38. Crystallization and Preliminary Crystallographic Analysis of Short-Chain Dehydrogenase/Reductase (SDR) from Thermus thermophilus HB8

Tomoyasu Kichise1, Kazuki Takeda1, Tamao Hisano1, Kunio Miki1,2
1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)


39. Crystal structure of the C subunit of V-type ATPase from Thermus thermophilus HB8

Nobutaka Numoto1, Akiko Kita1, Kunio Miki1,2
1Grad. Sch. Sci., Kyoto Univ., 2RIKEN Harima Inst.)


40. Inhibition of Thermus thermophilus HB8 Thioredoxin Activity by Platinum(II) Complex

Masahiro Kato1, Hitoshi Yamamoto1,2, Taka-aki Okamura1, Ryoji Masui1,2, Seiki Kuramitsu1,2, Norikazu Ueyama1
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


42. Structural analysis of mercuric reductase from Thermus thermophilus HB8

Hiroshi Hashimoto, Naoki Shichijo, Toshiyuki Shimizu, Mamoru Sato
(Grad. Sch. Integrated Sci., Yokohama City Univ.)


43. Crystal structure of P-protein of the glycine cleavage system from Thermus thermophilus HB8

Tadashi Nakai1,2, Noriko Nakagawa1,2, Nobuko Maoka1, Ryoji Masui1,2, Seiki Kuramitsu1,2, Nobuo Kamiya1
1RIKEN Harima Inst., 2Grad. Sch. Sci., Osaka Univ.)


44. Crystal structure of putative phosphomannomutase from Thermus thermophilus HB8

Shintaro Misaki1, Kenji Suzuki1, Naoki Kunishima2, Mitsuaki Sugawara2, Chizu Kuroishi2, Masanori Kobayashi1, Shoko Fujimoto1, Masahiro Sakurai1, Kazumi Nishijima1
1Joint Research Group, Pharmaceutical Consortium for Protein Structure Analysis (PcProt), 2RIKEN Harima Inst.)


45. Crystallographic analysis of malate dehydrogenase from thermophilic bacterium Thermus flavus and the mutant EX7 to elucidate the molecular mechanisms of their cofactor specificities and catalytic properties

Takeo Tomita1, Shinya Fushinobu2, Makoto Nishiyama1
1Biothch. Res. Center., Tokyo Univ., 2Grad. Sch. Biotech., Tokyo Univ.)


46. Biochemical and crystallographic analyses of carboxypeptidase 1 from Thermus thermophilus

Shiho TSUTSUI1, Woo Cheol LEE1, Kosuke ITO1, Masayuki KAMO1, Yumiko INOUE2, Koji NAGATA1, Masaru TANOKURA1,2
1Grad. Sch. Agr. and Life Sci., Tokyo Univ., 2RIKEN Harima Inst.)


47. The crystal structure of peptide deformylase from Thermus thermophilus HB8

Masayuki Kamo1, Norio Kudo1, WooCheol Lee1, Hiroyuki Motoshima2, Masaru Tanokura1,2
1Grad. Sch. Agr. and Life Sci. Tokyo Univ., 2RIKEN Harima Inst.)


48. X-Ray Analysis of Peptideglycan Synthetase family from Thermus thermophilus HB8

Shohei Kobayashi1, Fujio Matsuzaki1, Ryoko Hirooka1, Makoto Kimura1,2, Yoshimitsu Kakuta1,2
1Grad. Sch. Agr., Kyushu Univ., 2RIKEN Harima Inst.)


49. Crystallographic studies of an ABC-ATPase (TT2939) from T. thermophilus HB8

Kazuki Takeda1, Kunio Miki1,2
1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)


50. Crystal structure of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8

Wakana Iwasaki1, Hideyuki Miyatake1 and Kunio Miki1,2
1RIKEN Harima Inst., 2Grad. Sch. Sci., Kyoto Univ.)


70. MutS2 possesses a nuclease activity promoted by MutL and MutS

Kenji FUKUI1, Ryoji MASUI1,2, and Seiki KURAMITSU1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


71. Nucleotide excision repair system of Thermus thermophilus HB8

Ikumi SAITO1, Noriko NAKAGAWA2, Ryoji MASUI1,2, Seiki KURAMITSU1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


72. X-ray Crystallographic Analysis of Endonuclease V from Thermus thermophilus HB8

Hitomi Yamaguchi, Kei Wada, Atsushi Yamagata, Yasuhiro Takahashi and Keiichi Fukuyama
(Grad. Sch. Sci., Osaka Univ.)


76. The Crystal Structure of Thermus thermophilus HB8 hypothetical protein TT1253

Chizuko Sasaki1, Ikuko Sugiura1, Shigetoshi Sugio1, Takashi Tamura2,3, Kenji Inagaki2,3
1Mitsubishi Chem. Co., 2RIKEN Harima Inst., 3Grad. Sch.Natural Sci. and Tech., Okayama Univ.)


77. Thermus thermophilus TT1383 has a novel dNTP triphosphohydrolase activity stimulated by dATP and dTTP

Naoyuki Kondo1, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


78. Mutational analysis of ADP-ribose pyrophosphatase from Thermus thermophilus HB8

Takushi Ooga1, Sachiko Yoshiba1, Noriko Nakagawa1, Ryoji Masui1,2 and Seiki Kuramitsu1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


79. Thermus thermophilus HB8 Ndx3 protein is a diadenosine tetraphosphate (Ap4A) hydrolase that is a member of Nudix family

Takashi Nishikubo1, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Grad. Sch. Sci., Osaka Univ., 2RIKEN Harima Inst.)


80. Biochemical characterization of Ndx2 protein, a member of Nudix family from Thermus thermophilus HB8

Taisuke Wakamatsu1, Takushi Ooga2, Noriko Nakagawa2, Ryoji Masui2,3 and Seiki Kuramitsu1,2,3
1Grad. Sch. Frontier Biosci., Osaka Univ ., 2Grad. Sch. Sci., Osaka Univ ., 3RIKEN Harima Inst.)


81. Substrate specificity of the nudix hydrolase Ndx7 from Thermus thermophilus HB8

Daisuke Yamagishi1, Ryoji Masui1,2 and Seiki Kuramitsu1,2
1Grad. Sch. Sci., Osaka Univ. 2RIKEN Harima Inst.)


82. Analysis of Polyamine Biosynthetic Pathway of the Thermus thermophilus

Mio Ohnuma1, Yusuke Terui1, Mizue Nagai1, Hiromi Ito1, Aya Chitose1, Masatada Tamakoshi1, Shin-ichi Yokobori1, Masaru Niitsu2, Keijiro Samejima2, Tairo Oshima1
(1Sch. Life Sci., Tokyo Univ Pharm. Life Sci., 2Sci. Facl. Pharm., Josai Univ.)


83. Crystal structures of the signal transducing protein GlnK from Thermus thermophilus HB8

Hiroaki Sakai1, Hongfei Wang1, Chie Takemoto-Hori1, Tatsuya Kaminishi1,2, Hiroto Yamaguchi1, Yuki Kamewari1, Takaho Terada1,3, Seiki Kuramitsu1,3,4, Mikako Shirouzu1,4 and Shigeyuki Yokoyama1,2,4
(1RIKEN GSC, 2Grad. Sch. Sci., Tokyo Univ., 3Grad. Sch. Sci., Osaka Univ., 4RIKEN Harima Inst.)